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| Pinhal,Danillo; Gadig,Otto BF; Wasko,Adriane P; Oliveira,Claudio; Ron,Ernesto; Foresti,Fausto; Martins,Cesar. |
| Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chondrichthyes; PCR; Species identification; 5S rDNA; Sharks. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200033 |
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| Nirchio,Mauro; Oliveira,Claudio; Ferreira,Irani Alves; Granado,Angel; Ron,Ernesto. |
| The karyotype and chromosomal characteristics of the characid fish Triportheus venezuelensis were investigated using differential staining techniques (C-banding, Ag-NOR staining) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number (2n = 52), karyotype composition and sex chromosome determination system of the ZZ/ZW type were the same as previously described in other species of the genus Triportheus. However, extensive variation regarding nucleolus organizer regions (NOR) different from other species was observed. 18S rDNA sequences were distributed on nine chromosome pairs, but the number of chromosomes with Ag-NORs was usually lower, reaching a maximum of four chromosomes. When sequential staining experiments... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 18S rDNA; Ag-NORs; C-band; FISH; Fish cytogenetics; Sex chromosomes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100007 |
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